multi-analyte inflammatory cytokine elisarray kit  (Qiagen)

Journal: Cells

Article Title: Signalling Pathways of Inflammation and Cancer in Human Mononuclear Cells: Effect of Nanoparticle Air Pollutants

doi: 10.3390/cells13161367

Figure Lengend Snippet: The effect of carbon black (CB), urban dust (UD), and nanoparticulate carbon black (NPCB) on cell membrane damage (( A ); lactate dehydrogenase (LDH) release), oxidative stress ( B ), DNA damage ( C ), and phagocytosis ( D ) in human monocytes (M), and monocyte-derived macrophages (MDM). The inflammatory response (TNFα; ( E )) to lipopolysaccharide (100 ng/mL for 24 h) is also included ( F ). All PMs were used at 100 µg/mL and the incubation time was 24 h. Cell transition was induced by phorbol-12-myristate 13-acetate (PMA; 100 nM) applied for 72 h. LDH was measured with CyQUANT LDH Cytotoxicity Assay kit, DNA ploidy was quantified using propidium iodide staining, intracellular oxidative stress was measured using 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) while phagocytosis was assessed with fluorescent beads (Cayman Phagocytosis kit). Fluorescence was quantified with flow cytometry. TNFα was measured in the culture medium using a Multi-Analyte Inflammatory Cytokine ELISArray Kit (Quiagen, Wroclaw, Poland). Data are means ± SD of 6–10 assays. * p < 0.05; ** p < 0.01 for comparisons with the corresponding control cells; ^^ p < 0.01 for comparisons with corresponding naive cells; # p < 0.05 for comparisons with LPS-treated cells.

Article Snippet: TNFα was quantified in the culture medium of M and MDM with the Multi-Analyte Inflammatory Cytokine ELISArray Kit (Qiagen, Manchester, UK).

Techniques: Membrane, Derivative Assay, Incubation, CyQUANT Assay, LDH Cytotoxicity Assay, Staining, Fluorescence, Flow Cytometry, Control

Journal: eLife

Article Title: An unexpected role of neutrophils in clearing apoptotic hepatocytes in vivo

doi: 10.7554/eLife.86591

Figure Lengend Snippet: ( A ) Cytokines secreted by normal nonapoptotic and apoptotic NCTC cells are not significantly changed, including IL-1α, IL-2, IL-4, IL-10, TNF-α, IFN-γ, and GM-CSF. ( B ) Immunoblots of target proteins (IL-1b, IL-8, IL-6, IL-12 receptors) in nontreated (Ctrl) and RNAi-treated HL60 cells. RNAi knockdown efficiency ranges from 70% to 90%. ( C ) Cell surface receptors in apoptotic and nonapoptotic NCTC cells, HEK293 cells, or HUVECs. Data are from ( A, C ) or representative of ( B ) three independent experiments (mean and sem in A, C ). Figure 4—figure supplement 1—source data 1. Immunoblots of target proteins (IL-1b, IL-8, IL-6, IL-12 receptors) in nontreated (Ctrl) and RNAi-treated HL60 cells.

Article Snippet: Cytokines secreted from nonapoptotic and apoptotic NCTC cells were screened with the Human Inflammatory Cytokines Multi-Analyte ELISArray Kit (QIAGEN).

Techniques: Western Blot, Knockdown

Journal: eLife

Article Title: An unexpected role of neutrophils in clearing apoptotic hepatocytes in vivo

doi: 10.7554/eLife.86591

Figure Lengend Snippet:

Article Snippet: Cytokines secreted from nonapoptotic and apoptotic NCTC cells were screened with the Human Inflammatory Cytokines Multi-Analyte ELISArray Kit (QIAGEN).

Techniques: Recombinant, shRNA, Plasmid Preparation, In Situ, Enzyme-linked Immunosorbent Assay, Software, Imaging, Light Microscopy